en Research (Original) Ruta graveolens locally known as ''Iranian sodab or sadab '' (Rutaceae), is a valuable medicinal and culinary plant that grows in Mediterranean zone and north of Iran. The plant is a real factory to produce secondary metabolites such as alkaloids, flavonoids, lignins, glycosides, coumarins and phenolics compounds. The present study investigated effect of salicylic acid on phenols and flavonoids content in callus culture of R. graveolens. Among different PGRs used in this study, MS media containing concentration of NAA (3 mg/l) are the most effective one for callus induction from internode explans. The results revealed that salicylic acid (0.5 mg/ml) showed as maximum as 10.65-fold improvement in total phenolic content (225.32 mg gallic acid equivalent/g of extract powder) and salicylic acid (20 mg/ml) showed 1.98-fold enhancement in total phenolic content (438.75 mg gallic acid equivalent/g of extract powder). Flavonoids content decreased at lower concentration of salicylic acid treatment (8.32 quercetin equivalent/g of extract powder) but increased at higher concentration (29.41 mg/g). From this study, it is concluded that salicylic acids can be used as elicitors to enhance the secondary metabolites in callus culture of R. graveolens. This study shows that the elicitation depends on elicitor dose and type of compound to be elicited. Salicylic Acid, Phenolic Content, Flavonoids Content, Callus Culture, Ruta Graveolens 32 36 http://tbsrj.mazums.ac.ir/browse.php?a_code=A-10-153-1&slc_lang=en&sid=1 Yaser Sharifi 100319475328460028873 100319475328460028873 No Msc. Department of Agricultural Biotechnology. Sari Agricultural Sciences and Natural Resources University, Sari, Iran Gorban Ali Nematzadeh 100319475328460028874 100319475328460028874 No Professor, Sari Agricultural Sciences And Natural Resources University, Sari, Iran. Valiollah Ghasemi Omran 100319475328460028875 100319475328460028875 No Assistant Professor, Genetics And Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences And Natural Resources University, Sari, Iran. Toktam Sadat Tavabe Ghavami 100319475328460028876 100319475328460028876 No Department of Genetics, Islamic Azad University, Science & Research Branch, Iran. Mohammad Ali Ebrahimzadeh zadeh20@gmail.com 100319475328460028877 100319475328460028877 Yes Professor, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.