other Adolescent Medicine Research (Original) Ruta graveolens, locally called sodab or sadab, is a known medicinal plant in north of Iran. The plant is a source for the production of secondary metabolites such as Glycosides, lignins, alkaloids, Coumarins, flavonoids and phenolic acids. In this work, the effect of salicylic acid on phenols and flavonoids contents of R. graveolens were investigated in the cell suspension culture. A significant enhancement in total phenolic and flavonoids contents were observed in salicylic acid treated samples. The results revealed that salicylic acid (10 mg/ml) showed as maximum as 3.14 fold enhancement in total flavonoid content (40.35 mg/g) and salicylic acid (20 mg quercetin equivalent/g of extract powder) showed as maximum as 18.33 fold improvement in total phenolic content (438.75 mg gallic acid equivalent/g of extract powder). Also in this study, salicylic acid (10 mg/ml) showed as maximum as 3.55 fold increase in DPPH radical-scavenging activity compared to control. These results suggest that the positive effect of salicylic acid on phenols and flavonoids content and DPPH scavenging activity in cell suspension culture. This effect was dose dependent Salicylic Acid, Phenolic Content, Flavonoids, DPPH, Cell Suspension Culture, Ruta Graveolens, Sodab 18 21 http://tbsrj.mazums.ac.ir/browse.php?a_code=A-10-3474-3&slc_lang=other&sid=1 Yaser Sharifi 100319475328460028994 100319475328460028994 No Msc. Department of Agricultural Biotechnology. Sari Agricultural Sciences and Natural Resources University, Sari, Iran. Valiollah Ghasemi Omran 100319475328460028995 100319475328460028995 No Assistant Professor, Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences And Natural Resources University,Sari, Iran Toktam Sadat Tavabe Ghavami 100319475328460028996 100319475328460028996 No Department of Genetics, Islamic Azad University, Science & Research Branch, Iran Gorban Ali Nematzadeh Gharakhili 100319475328460028997 100319475328460028997 No Professor, Sari Agricultural Sciences And Natural Resources University, Sari, Iran. Mohammad Ali Ebrahimzadeh zadeh20@gmail.com 100319475328460028998 100319475328460028998 Yes Professor, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran