Tabari Biomedical Student Research Journal

en Investigating the Combinational Effect of Micellar Curcumin and Metformin on the Invasion of PC3 Prostate Cancer Cell Line Biotechnology Research (Original) Introduction: Adverse side effects, non-specific function, and increasing tolerability of tumor cells to chemotherapy demand therapeutic strategies with fewer side effects and better efficacy. Curcumin and metformin have been shown to have anticancer properties, according to emerging research. Therefore, micellar curcumin and metformin were combined and analyzed on cancer cell line PC3. Material and Methods: Cell viability after drug treatment, individually and in combination, was evaluated using an MTT assay. In order to determine the type of interaction between micellar curcumin and metformin, the median-effect method was employed. Subsequently, the expression of MMP-2 and MMP-9 of cells treated with single drugs and a synergistic combination of compounds was assessed using Gelatin Zymography test. Moreover, evaluating the number of invasive cells under treatment with drugs alone and in combination through matrigel invasion assay. Results: The results demonstrated that the cell proliferation treated with micellar curcumin and metformin alone and in combination decreased significantly (P<0.05) during 24 and 48 h. Besides, co-treatment of compounds showed synergy within 48 h. Although all the cells treated, whether with single drugs or the synergistic combination of both, showed a decline in expressing MMP-2, MMP-9, and the number of migrated cells, the maximum reduction was seen when micellar curcumin and metformin were combined. Conclusion: This study showed that metformin and micellar curcumin together have a synergistic effect on cell proliferation inhibition in the PC3 cell line. Metformin and micellar curcumin were more effective as a combination in decreasing the PC3 cell line invasion by reducing MMP-2 and MMP-9. Combination therapy, Drug repositioning, Invasion assay, Metformin, Micellar curcumin, Prostate cancer 1 12 http://tbsrj.mazums.ac.ir/browse.php?a_code=A-10-3574-1&slc_lang=en&sid=1 Nazanin Ghahari nazanin.ghahari.92@gmail.com 100319475328460031227 100319475328460031227 No Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Shadi Abkhiz abkhiz.sh@iums.ac.ir 100319475328460031228 100319475328460031228 No Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran Hamed Montazeri montazerighods.h@iums.ac.ir 100319475328460031229 100319475328460031229 No Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Iran University of Medical Sciences, Tehran, Iran Parastoo Tarighi tarighi.p@iums.ac.ir 100319475328460031230 100319475328460031230 Yes Department of Medical Biotechnology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran