Volume 8, Issue 2 (9-2026)
Tabari Biomed Stu Res J 2026, 8(2): 33-42 |
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rezaie S, hashemi juybari F. Cold Plasma Effects on Seed Priming of Sage (Salvia officinalis L.). Tabari Biomed Stu Res J 2026; 8 (2) :33-42
URL: http://tbsrj.mazums.ac.ir/article-1-3914-en.html
URL: http://tbsrj.mazums.ac.ir/article-1-3914-en.html
Abstract:
Cold plasma is an emerging physical technology for seed priming; however, its effects on sage (Salvia officinalis L.) remain poorly characterized. This study investigated the effects of dielectric barrier discharge (DBD) argon plasma on germination indices, seedling growth, and biochemical traits of sage seeds. A completely randomized design with four treatments (control, 3, 5, and 10 min plasma exposure) and three replications was employed.
Plasma treatment significantly (p < 0.001) enhanced all germination traits. Germination percentage increased from 75.67% (control) to 94.00% (10 min), while germination rate rose from 3.20 to 4.90. Mean germination time decreased from 6.20 to 4.00 days. Radicle and plumule lengths increased by 92% and 64%, respectively, at 10 min. Soluble carbohydrates and total proteins reached their maximum values at 5 min (17.47 and 11.20 mg/g FW), showing 44% and 43% increases over the control. Vegetative growth parameters, including root length, shoot length, and leaf number, were also maximized at 5 min.
Seedling vigor index showed a remarkable increase from 16.74 (control) to 45.08 (10 min), representing a 2.7-fold improvement. The 5-min treatment was optimal for vegetative growth and biochemical accumulation, while 10-min was superior for germination traits. These improvements are attributed to enhanced seed coat permeability, increased enzyme activity, and improved reserve mobilization induced by plasma-generated reactive species. Therefore, cold plasma is proposed as a non-chemical, eco-friendly priming method for medicinal plant cultivation.
Plasma treatment significantly (p < 0.001) enhanced all germination traits. Germination percentage increased from 75.67% (control) to 94.00% (10 min), while germination rate rose from 3.20 to 4.90. Mean germination time decreased from 6.20 to 4.00 days. Radicle and plumule lengths increased by 92% and 64%, respectively, at 10 min. Soluble carbohydrates and total proteins reached their maximum values at 5 min (17.47 and 11.20 mg/g FW), showing 44% and 43% increases over the control. Vegetative growth parameters, including root length, shoot length, and leaf number, were also maximized at 5 min.
Seedling vigor index showed a remarkable increase from 16.74 (control) to 45.08 (10 min), representing a 2.7-fold improvement. The 5-min treatment was optimal for vegetative growth and biochemical accumulation, while 10-min was superior for germination traits. These improvements are attributed to enhanced seed coat permeability, increased enzyme activity, and improved reserve mobilization induced by plasma-generated reactive species. Therefore, cold plasma is proposed as a non-chemical, eco-friendly priming method for medicinal plant cultivation.
Keywords: Cold plasma, Seed priming, Salvia officinalis L., Germination, Biochemical traits, Seedling vigor
Type of Study: Research (Original) |
Subject:
Biology
Published: 2026/09/19 | ePublished: 2026/09/19
Published: 2026/09/19 | ePublished: 2026/09/19
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