Volume 1, Issue 1 (3-2019)
Tabari Biomed Stu Res J 2019, 1(1): 22-27 |
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Rouhi S, Shakib P, Heydarifar M, Ramazanzadeh R. Survey of hospital environment regarding the presence of Staphylococcus aureus producing β-lactamase. Tabari Biomed Stu Res J 2019; 1 (1) :22-27
URL: http://tbsrj.mazums.ac.ir/article-1-3613-en.html
URL: http://tbsrj.mazums.ac.ir/article-1-3613-en.html
Survey of hospital environment regarding the presence of Staphylococcus aureus producing β-lactamase
1- Lung Diseases and Allergy Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
2- Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3- Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
4- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
2- Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3- Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
4- Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract:
Staphylococcus aureus is a common cause of hospital-acquired infections. This bacterium is resistant to β-lactam antibiotics. The aim of this study was to evaluate the prevalence of S. aureus strains producing enzymes capable of destroying β-lactam antibiotics in a hospital environment. This descriptive and analytic study was performed in Besat Hospital, Sanandaj, Iran, 2016, on 158 swabs collected from the hospital environment. The S. aureus bacteria were identified by microbiology methods, and β-lactamase was determined using acidometric and combined disk methods, as well as Hodge test. Data analysis was conducted using Chi-squared and two-way analysis of variance in SPSS software, version 16 (P<0.05). Out of 201 isolated bacteria, 74.68% were S. aureus. Regarding the results of acidometric method, 22.88% of the strains was β-lactamase producing. In combined disc method, 5.08% of strains produced Metallo-β-lactamase; and according to the Hodge test, 84.50% showed carbapenemases. In places with repetitive cleaning, fewer isolates of bacteria were isolated (P<0.05). There was no significant difference between the results of the three methods (P>0.05). In this study, the β-lactamase producing strains of S. aureus isolates were observed in the hospital. Therefore, the periodic monitoring of resistance and using appropriate detergents are very important at hospitals.
Keywords: Staphylococcus aureus, Phenotypic method, Enzymic degradation of β-lactam antibiotics, Hospital environment
Type of Study: Research (Original) |
Published: 2019/03/15 | ePublished: 2019/03/15
Published: 2019/03/15 | ePublished: 2019/03/15
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