Volume 1, Issue 3 (11-2019)
Tabari Biomed Stu Res J 2019, 1(3): 32-36 |
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Sharifi Y, Nematzadeh G A, Ghasemi Omran V, Tavabe Ghavami T S, Ebrahimzadeh M A. Effect of salicylic acid on phenols and flavonoids content in callus culture of Iranian sodab (Ruta graveolens): A threatened medicinal plant of north of Iran. Tabari Biomed Stu Res J 2019; 1 (3) :32-36
URL: http://tbsrj.mazums.ac.ir/article-1-3687-en.html
URL: http://tbsrj.mazums.ac.ir/article-1-3687-en.html
Yaser Sharifi1 
, Gorban Ali Nematzadeh2 , Valiollah Ghasemi Omran3 , Toktam Sadat Tavabe Ghavami4 , Mohammad Ali Ebrahimzadeh * 
5
, Gorban Ali Nematzadeh2 , Valiollah Ghasemi Omran3 , Toktam Sadat Tavabe Ghavami4 , Mohammad Ali Ebrahimzadeh * 5
1- Msc. Department of Agricultural Biotechnology. Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2- Professor, Sari Agricultural Sciences And Natural Resources University, Sari, Iran.
3- Assistant Professor, Genetics And Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences And Natural Resources University, Sari, Iran.
4- Department of Genetics, Islamic Azad University, Science & Research Branch, Iran.
5- Professor, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.
2- Professor, Sari Agricultural Sciences And Natural Resources University, Sari, Iran.
3- Assistant Professor, Genetics And Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences And Natural Resources University, Sari, Iran.
4- Department of Genetics, Islamic Azad University, Science & Research Branch, Iran.
5- Professor, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract:
Ruta graveolens locally known as ''Iranian sodab or sadab '' (Rutaceae), is a valuable medicinal and culinary plant that grows in Mediterranean zone and north of Iran. The plant is a real factory to produce secondary metabolites such as alkaloids, flavonoids, lignins, glycosides, coumarins and phenolics compounds. The present study investigated effect of salicylic acid on phenols and flavonoids content in callus culture of R. graveolens. Among different PGRs used in this study, MS media containing concentration of NAA (3 mg/l) are the most effective one for callus induction from internode explans. The results revealed that salicylic acid (0.5 mg/ml) showed as maximum as 10.65-fold improvement in total phenolic content (225.32 mg gallic acid equivalent/g of extract powder) and salicylic acid (20 mg/ml) showed 1.98-fold enhancement in total phenolic content (438.75 mg gallic acid equivalent/g of extract powder). Flavonoids content decreased at lower concentration of salicylic acid treatment (8.32 quercetin equivalent/g of extract powder) but increased at higher concentration (29.41 mg/g). From this study, it is concluded that salicylic acids can be used as elicitors to enhance the secondary metabolites in callus culture of R. graveolens. This study shows that the elicitation depends on elicitor dose and type of compound to be elicited.
Type of Study: Research (Original) |
Published: 2019/10/26 | ePublished: 2019/10/26
Published: 2019/10/26 | ePublished: 2019/10/26
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