Volume 7, Issue 2 (12-2025)
Tabari Biomed Stu Res J 2025, 7(2): 25-32 |
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Jafari-Sales A, Golestani A, Ghahremani Z, Farzipour M, Jalilpouri A, Pourmohebbi S, et al . Antibiotic Resistance and Prevalence of blaOXA-48 and blaGES-2 Genes in Pseudomonas aeruginosa Isolated from Burn Patients in Tabriz, Iran. Tabari Biomed Stu Res J 2025; 7 (2) :25-32
URL: http://tbsrj.mazums.ac.ir/article-1-3893-en.html
URL: http://tbsrj.mazums.ac.ir/article-1-3893-en.html
Abolfazl Jafari-Sales1 
, Aylin Golestani2 , Zahra Ghahremani2 , Mahsa Farzipour3 , Ayda Jalilpouri3 , Shayan Pourmohebbi3 , Ghazal Kadkhodaei-Ilkhechi2 , Maryam Farahnaki-Sadabadi2 , Mehrdad Pashazadeh *4
, Aylin Golestani2 , Zahra Ghahremani2 , Mahsa Farzipour3 , Ayda Jalilpouri3 , Shayan Pourmohebbi3 , Ghazal Kadkhodaei-Ilkhechi2 , Maryam Farahnaki-Sadabadi2 , Mehrdad Pashazadeh *4
1- Department of Microbiology, Kaz.C., Islamic Azad University, Kazerun, Iran.
2- Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran.
3- Department of Biotechnology, Ta.C., Islamic Azad University, Tabriz, Iran
4- Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran
2- Department of Cellular and Molecular Biology, Ta.C., Islamic Azad University, Tabriz, Iran.
3- Department of Biotechnology, Ta.C., Islamic Azad University, Tabriz, Iran
4- Department of Laboratory Sciences and Microbiology, TaMS.C., Islamic Azad University, Tabriz, Iran
Abstract:
Introduction:
Pseudomonas aeruginosa (P. aeruginosa) is a major cause of hospital-acquired infections in burn units, and its antibiotic resistance presents a significant therapeutic challenge. The emergence of carbapenemase genes, such as blaOXA-48 and blaGES-2, is of particular concern. This study aimed to determine the antibiotic resistance patterns and the prevalence of blaOXA-48 and blaGES-2 genes in P. aeruginosa isolates from burn patients in Tabriz, Iran.
Methods and Materials:
In this cross-sectional study, 100 clinical isolates of P. aeruginosa were collected from burn patients between June and January 2025. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method according to clinical and laboratory standards institute guidelines. Phenotypic detection of extended-spectrum beta-lactamases (ESBL) was conducted using the combined disk test. The presence of blaOXA-48 and blaGES-2 genes was confirmed by polymerase chain reaction.
Results:
The highest resistance rates were observed against ceftazidime and cefotaxime, each at 96%, followed by imipenem at 91% and meropenem at 90%. Phenotypically, 50% of the isolates were identified as ESBL producers. Genotypic analysis of ESBL-positive isolates revealed that 62% (31/50) carried the blaGES-2 gene, while 28% (14/50) harbored the blaOXA-48 gene. Concurrent presence of both genes was detected in 12% (6/50) of the ESBL-positive isolates.
Conclusion:
The results demonstrated a high prevalence of antibiotic resistance and a concerning presence of carbapenemase genes blaGES-2 and blaOXA-48 in P. aeruginosa isolates from burn patients in Tabriz. This highlights the urgent need for continuous surveillance programs and enhanced infection control strategies in burn care centers.
Pseudomonas aeruginosa (P. aeruginosa) is a major cause of hospital-acquired infections in burn units, and its antibiotic resistance presents a significant therapeutic challenge. The emergence of carbapenemase genes, such as blaOXA-48 and blaGES-2, is of particular concern. This study aimed to determine the antibiotic resistance patterns and the prevalence of blaOXA-48 and blaGES-2 genes in P. aeruginosa isolates from burn patients in Tabriz, Iran.
Methods and Materials:
In this cross-sectional study, 100 clinical isolates of P. aeruginosa were collected from burn patients between June and January 2025. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method according to clinical and laboratory standards institute guidelines. Phenotypic detection of extended-spectrum beta-lactamases (ESBL) was conducted using the combined disk test. The presence of blaOXA-48 and blaGES-2 genes was confirmed by polymerase chain reaction.
Results:
The highest resistance rates were observed against ceftazidime and cefotaxime, each at 96%, followed by imipenem at 91% and meropenem at 90%. Phenotypically, 50% of the isolates were identified as ESBL producers. Genotypic analysis of ESBL-positive isolates revealed that 62% (31/50) carried the blaGES-2 gene, while 28% (14/50) harbored the blaOXA-48 gene. Concurrent presence of both genes was detected in 12% (6/50) of the ESBL-positive isolates.
Conclusion:
The results demonstrated a high prevalence of antibiotic resistance and a concerning presence of carbapenemase genes blaGES-2 and blaOXA-48 in P. aeruginosa isolates from burn patients in Tabriz. This highlights the urgent need for continuous surveillance programs and enhanced infection control strategies in burn care centers.
Type of Study: Research (Original) |
Subject:
Biotechnology
Published: 2025/12/19 | ePublished: 2025/12/19
Published: 2025/12/19 | ePublished: 2025/12/19
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